OPTIMIZATION OF SEED STERILIZATION AND IN VITRO CULTIVATION METHODS FOR EFFECTIVE MICROPROPAGATION OF ARNICA MONTANA L.
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Szerzők: Cím: OPTIMIZATION OF SEED STERILIZATION AND IN VITRO CULTIVATION METHODS FOR EFFECTIVE MICROPROPAGATION OF ARNICA MONTANA L. DOI: https://doi.org/10.71066/FTTMK.1.01 Megjelent: 2025. december
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ABSTRACT
Arnica montana is an invaluable medicinal herb, but its wild populations are declining. To aid conservation as well as propagation, the present work investigated methods for sterilizing seeds and culture media for initiating in vitro growth. Seeds sourced in the Ukrainian region surrounding the Krasna mountain range were sterilized by employing different chemicals; sodium hypochlorite gave the most regular and healthy-looking explants. Of the nutrient media tested, Murashige and Skoog (MS) furnished the highest initial development. In IAA-BAP combinations, the best formation was tested under different concentrations. Balanced levels, especially 1.0 mg/L of both growth regulators, resulted in robust as well as regular shooting. While high hormone levels decreased the viability, growth suppression was evident. The results point towards an easy and efficient protocol for starting cultures of A. montana applicable for both research purposes as well as practical cultivation.
KEYWORDS
medicinal plants, seed sterilization, micropropagation, hormone balance, tissue culture
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